a better way to acquire data on mass cytometers

 

The Super Sampler is a new fluidics system
for the CyTOF mass cytometer that gets you
more data, faster, without an operator.

 

Highest Recovery

Recover an average of twice the number of cells from your samples. Don't have many cells? Not a problem.

Above: 106,000 peripheral blood cells were loaded using the Super Sampler or the two CyTOF2 sample loops. Acquisition for sample loops was allowed to continue for 700 seconds, ensuring entirety of sample volume was loaded. Paired bars represent…

Above: 106,000 peripheral blood cells were loaded using the Super Sampler or the two CyTOF2 sample loops. Acquisition for sample loops was allowed to continue for 700 seconds, ensuring entirety of sample volume was loaded. Paired bars represent duplicate experiments.

Fastest Acquisition

Samples injected via the stock sample loops suffer from wildly varying event rates. At any given time, you're either above or below the maximum singlet event rate, wasting either cells or time. With the Super Sampler, the event rate stays within 5% of the optimum, allowing you to analyze the same number of cells in 40% less time.

Above: The same number of mouse splenocytes were loaded using the Super Sampler or the CyTOF1 sample loop. Total event rate (dotted line) and singlet event rate (solid line) were plotted over time. Singlets were identified using the method of Zunder…

Above: The same number of mouse splenocytes were loaded using the Super Sampler or the CyTOF1 sample loop. Total event rate (dotted line) and singlet event rate (solid line) were plotted over time. Singlets were identified using the method of Zunder and Finck (Nature Protocols  10, 316–333 (2015)). The cells were diluted into two different volumes for manual loading to illustrate the limited ability to maintain the optimal rate.

Large Samples

Big samples from multiplexed experiments are continuously loaded over the course of hours without an operator.  Any size is fine: 10 million cells, 10 ml,  you got it.  Don't worry about cells settling either.

Cloud-based monitoring alerts you if there's a problem while you're off doing better things than watching the instrument.

Small Samples

Samples below 500 µl still benefit from higher recovery and faster acquisition. Automatic cleaning between samples ensures low carry-over.

Save Time and Money

Faster acquisition makes it like having half of an additional cytometer.

Unattended acquisition of large samples eliminates the need for a technician to painstakingly attend to the instrument, or frees up your time to do other work.

Improved sample recovery means fewer cells required for analysis—for example, fewer mice needed for small organ preparations.

Cloud-Based Monitoring

Track your run status from anywhere. Get paged if the cytometer has a problem.

Turn your CyTOF into a workhorse.

Super Sampler

The better way to acquire data on a mass cytometer. Includes the Super Sampler hardware, software and one year of cloud-based monitoring, with free software upgrades.

Works with Fluidigim CyTOF 1 and CyTOF 2 mass cytometers.

Sample Chiller

The perfect companion to the Super Sampler. Keeps your sample cold and secure during runs, without the hassle and risk of your sample sinking into melted ice water.

Get more info

Fluidigm and CyTOF are registered trademarks of Fluidigm Corporation.